Mycotoxins
Instrumentation

Cannabis ChemLab harnesses the cutting-edge capabilities of the Agilent 1260/6470 LC/TQ system for the precise detection and quantification of mycotoxins, including Aflatoxin B1, Aflatoxin B2, Aflatoxin G1, Aflatoxin G2, and Ochratoxin A, in cannabis samples. This advanced analytical instrument integrates liquid chromatography (LC) with triple quadrupole mass spectrometry (TQ), synergizing the separation power of LC with the sensitive and selective detection capabilities of mass spectrometry.

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The analysis begins with meticulous sample preparation, where cannabis extracts are meticulously extracted and purified to isolate the target mycotoxins from the complex matrix of the sample. Following purification, the prepared sample is introduced into the LC system for separation. Within the LC system, the sample components undergo chromatographic separation based on their distinct chemical properties, allowing for individual mycotoxins to be resolved and eluted at different retention times.

Upon elution from the LC column, the separated analytes enter the mass spectrometer for detection and quantification. The Agilent 1260/6470 LC/TQ system features a triple quadrupole mass analyzer configuration, comprising three quadrupoles (Q1, Q2, and Q3), each with specific functions in the analytical process.

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In the first quadrupole (Q1), precursor ions corresponding to the target mycotoxins are selectively filtered based on their mass-to-charge ratio (m/z). These precursor ions are then directed to the collision cell situated between Q1 and Q2, where they undergo collision-induced dissociation (CID). During CID, the precursor ions are fragmented into smaller product ions.

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Subsequently, the fragmented product ions are guided into the third quadrupole (Q3), where they undergo further filtering based on their mass-to-charge ratio. By precisely selecting specific transitions between precursor and product ions for each mycotoxin, the LC/TQ system can effectively differentiate and quantify the target analytes within the sample.

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This targeted approach offers exceptional sensitivity and specificity, enabling the detection and quantification of mycotoxins at ultra-trace levels in cannabis samples. Furthermore, the LC/TQ system's robust performance ensures minimal interference from matrix components, thereby enhancing the reliability and accuracy of the analytical results.

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Overall, the Agilent 1260/6470 LC/TQ system represents a state-of-the-art solution for mycotoxin analysis in cannabis, empowering Cannabis ChemLab to deliver comprehensive and reliable testing services to ensure the safety and compliance of cannabis products.