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Gender Detection
Instrumentation

PCR-Phases
Cannabis Leaf

Quantitative Polymerase Chain Reaction

The Aria MX qPCR (quantitative Polymerase Chain Reaction) system is a sophisticated molecular tool used for amplifying and quantifying DNA, enabling precise genetic analysis, including sex determination in cannabis.

How the Aria MX qPCR Amplifies DNA:

1. DNA Extraction: Initially, DNA is extracted from cannabis seeds, seedlings, or plant tissues.

2. Preparation of the Reaction Mix: The extracted DNA is mixed with specific primers (short DNA fragments designed to bind to target sequences), a DNA polymerase enzyme, nucleotides, and other components essential for PCR.

3. Thermal Cycling:
   - Denaturation: The reaction mix is heated to separate the DNA strands, typically around 95°C.
   - Annealing: The temperature is lowered to allow the primers to bind (anneal) to specific sequences on the DNA strands. For sex determination in cannabis, primers specific to sequences found on the Y chromosome (male) or sequences common to both X and Y chromosomes are used.
   - Extension: The temperature is adjusted so the DNA polymerase can add nucleotides to the primed sequences, extending the DNA strand and creating new copies.

4. Quantification: The Aria MX system employs fluorescent markers that bind to the newly formed DNA strands. The fluorescence emitted is directly proportional to the amount of DNA generated, and it's measured in real-time during the PCR cycles. This process allows for the quantification of specific DNA sequences.

Application for Male/Female Detection in Cannabis:

In cannabis, the presence or absence of the Y chromosome determines the plant's sex (male or female). The Aria MX qPCR can rapidly amplify and quantify DNA segments that are unique to the Y chromosome. 

- Male Detection: If the primers specific to the Y chromosome regions produce a fluorescent signal, it indicates the presence of male genetics.


- Female Detection: Lack of fluorescence from Y chromosome-specific primers indicates a female plant, as females have two X chromosomes.

This method allows growers to determine the sex of cannabis plants at a very early stage, well before sexual characteristics become physically apparent. Early detection of male plants enables growers to segregate or remove them from cultivation areas, preventing unwanted pollination of female plants. This selective cultivation is crucial for producing high-quality, seedless cannabis flowers, maximizing yield and market value.

Cannabis Leaf
PCR amplification plot

How does Cannabis ChemLab Stand Out?

Cannabis ChemLab excels in the cannabis industry by offering specialized gender detection services. Using the latest qPCR technology, we accurately determine the sex of cannabis seeds, seedlings, and plants at an early stage. This precision allows cultivators to identify and remove male plants promptly, preventing unwanted pollination and ensuring that female plants produce high-quality, seedless buds. Our gender detection service is essential for growers looking to maximize yield and efficiency, making Cannabis ChemLab a trusted partner in achieving optimal cultivation results.

Instrumentation

The Aria Mx system identifies gender in cannabis by employing qPCR technology, which amplifies specific microbial DNA. As the DNA replicates, fluorescent probes attached to the target sequences emit light, which the Aria Mx measures. This process allows for precise quantification of gender, offering a highly accurate assessment of gender to ensure proper detection.

Agilent AriaMx

Medicinal Genomics

Medicinal Genomics is a pioneering company specializing in genetic solutions and testing technologies for the cannabis industry. It provides advanced tools for the identification and quantification of microbial contaminants in cannabis products.

 

Cannabis ChemLab leverages Medicinal Genomics' cutting-edge qPCR assays to test for specific analytes, including gender accurately.

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