top of page

Virus and Viroid
Instrumentation

PCR-Phases
Cannabis Leaf

Quantitative Polymerase Chain Reaction

Let's break down these processes step by step, focusing on their importance in cannabis testing for microbial contaminants.

DNA Isolation in Cannabis for Medicinal Genomics

DNA isolation from cannabis involves extracting the genetic material of any plant pathogens present on or in the plant. This process begins with breaking open the microbial cells (lysis) to release their DNA. This can involve physical methods like grinding, chemical methods using detergents to dissolve cell membranes, or enzymes to digest cellular components. The DNA is then separated from the rest of the cell debris through a series of purification steps. Medicinal Genomics utilizes specialized protocols and kits designed for cannabis samples, ensuring that the DNA isolated is of high quality and free from plant contaminants, ready for qPCR analysis.

Medicinal Genomics qPCR Assays for Detecting Microbial Contaminants

Quantitative Polymerase Chain Reaction (qPCR) assays by Medicinal Genomics leverage the isolated DNA to detect and quantify microbial contaminants. qPCR is a highly sensitive technique that amplifies specific DNA sequences of interest - in this case, sequences unique to microbial contaminants like bacteria, fungi, and plant pathogens. By using primers that are specific to the DNA of target microbes, the qPCR assay can rapidly amplify these sequences if they are present, making even small amounts of DNA detectable. Each cycle of the qPCR process doubles the amount of targeted DNA, and the accumulation of DNA is monitored in real-time, allowing for the quantification of microbial load in the sample.

Agilent Aria Mx Detection Using Fluorescent Probes

The Agilent Aria Mx utilizes fluorescent probes to detect the analyte of interest during qPCR assays. These probes are designed to attach to specific DNA sequences during the amplification process. Each probe contains a fluorescent dye, which only emits light when the probe is bound to its target DNA sequence and the DNA polymerase enzyme cleaves the probe during amplification. The Aria Mx instrument detects the intensity of the fluorescence emitted, which increases with each cycle of amplification, correlating directly with the amount of target DNA present.

 

By analyzing the fluorescence data, the Aria Mx can precisely quantify the level of plant pathogen contaminants in a cannabis sample, offering a powerful tool for ensuring grow facility maintenance and cannabis crop health. 

Together, these technologies and methods form a comprehensive approach to detecting and quantifying microbial contaminants in cannabis, ensuring product safety for medicinal and recreational use.

Cannabis Leaf
qPCR amplification plot

How does Cannabis ChemLab Stand Out?

Cannabis ChemLab distinguishes itself from the competition through its deep expertise in plant science and plant pathogens, coupled with state-of-the-art technology. With a team of experts specializing in various fields of plant biology and pathology, Cannabis ChemLab offers unparalleled insights into cannabis cultivation, disease management, and quality assurance. Utilizing cutting-edge laboratory equipment and innovative testing methodologies, Cannabis ChemLab delivers accurate and comprehensive results to ensure the safety, quality, and efficacy of cannabis products. Whether it's identifying and mitigating plant pathogens or optimizing cultivation practices, Cannabis ChemLab's commitment to excellence sets it apart as a trusted partner in the cannabis industry.

Instrumentation

The Aria Mx system identifies microbial contaminants in cannabis by employing qPCR technology, which amplifies specific microbial DNA. As the DNA replicates, fluorescent probes attached to the target sequences emit light, which the Aria Mx measures. This process allows for precise quantification of pathogen levels, offering a highly accurate assessment of contamination to ensure grow facility success.

Agilent Aria Mx qPCR

Medicinal Genomics

Cannabis ChemLab harnesses the power of Medicinal Genomics assays to empower growers in safeguarding their cannabis crops against prevalent plant pathogens.

 

By leveraging advanced genetic testing technologies developed by Medicinal Genomics, Cannabis ChemLab provides growers with valuable insights into the presence of harmful pathogens such as fungi, bacteria, and viruses.

 

Through targeted assays designed to detect specific genetic markers associated with these pathogens, Cannabis ChemLab enables growers to implement proactive measures to prevent, manage, and control disease outbreaks.

 

By offering accurate and timely diagnostic information, Cannabis ChemLab empowers growers to make informed decisions that optimize crop health, mitigate risks, and maximize yields.

bottom of page